mmp7 protein  (Proteintech)

Journal: International Journal of Molecular Sciences

Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

doi: 10.3390/ijms27052209

Figure Lengend Snippet: Increased MMP7 in BA patients correlated with the progression of liver fibrosis. Serum and liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Serum MMP7 levels were determined by ELISA. ( B ) Relative MMP7 mRNA levels in livers of BA and non-BA patients. ( C ) Representative images of immunostaining for MMP7 and quantitative analysis of MMP7 staining in liver tissue. Magnification: left panel: ×100; right panel: ×400. ( D ) Representative Masson trichrome staining images of METAVIR fibrosis stages (F1–F4) in BA patients. Scale bar: 400 μm. ( E – G ) Serum MMP7 levels, relative MMP7 mRNA levels and the expression intensity of MMP7 in liver of BA patients with moderate (F0–F2) or advanced liver fibrosis (F3–F4). ( H ) Spearman’s rank correlation analysis of MMP7 expression level with fibrotic marker genes, including COL1A1 , ACTA2 , TGFB1 , and TIMP1 .

Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Immunostaining, Staining, Expressing, Marker

Journal: International Journal of Molecular Sciences

Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

doi: 10.3390/ijms27052209

Figure Lengend Snippet: MMP7 gene correlation analysis and pathway enrichment in BA. ( A ) Distribution of correlation coefficients between MMP7 expression and all other genes across two independent BA datasets ( GSE15235 and GSE46960 ). ( B ) GSEA results using gene lists ranked by correlation with MMP7.

Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

doi: 10.3390/ijms27052209

Figure Lengend Snippet: EMT scores were associated with MMP7, liver fibrosis and survival in BA patients. Liver samples of patients with non-BA ( n = 39) disease and BA ( n = 66) were collected. ( A ) Representative image of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in liver biopsies samples. Magnification: left panel : ×100; right panel : ×400. ( B ) Percentage of samples according to the intensity and extension of EMT-related protein immunostaining in cholangiocytes from two groups. ( C ) EMT scores of cholangiocytes in non-BA and BA patients. ( D ) EMT scores in two subgroups divided by MMP7 expression intensity in BA patients. ( E ) Correlation analysis of fibrosis stage EMT scores in BA patients. Spearman’s rank correlation.

Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

Techniques: Immunostaining, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

doi: 10.3390/ijms27052209

Figure Lengend Snippet: MMP7 promoted EMT in an E-cadherin/β-catenin pathway-dependent manner. ( A ) Protein expression of E-cadherin in HIBEpiCs after treatment with different concentrations of MMP7 for 24 h. ( B ) Level of sE-cadherin in the cell culture supernatant of HIBEpiCs after MMP7 treatment. ( C ) Western blot analysis of β-catenin protein in the nucleus or cytoplasm of HIBEpiCs. ( D ) Immunofluorescence staining shows the subcellular localization of β-catenin in HIBEpiCs. Scale bar:50 μm. ( E ) TOP/FOP-Flash luciferase activity of MMP7-treated cells. ( F ) Effect of the MMP7 and ICG-001 on β-catenin downstream target genes expression (including SNAI1 , SNAI2 , MMP7 ) in HIBEpiCs. ( G , H ) Effect of the MMP7 and ICG-001 on mRNA and protein expression of EMT-related markers (including E-cadherin, Vimentin, S100A4) in HIBEpiCs. ( I ) ELISA analysis of serum level of sE-cadherin in patients. ( J ) Spearman’s rank correlation analysis of serum sE-cadherin and serum MMP7 in BA patients. ( K ) Representative images of immunostaining of β-catenin in cholangiocytes of non-BA and BA patients. Magnification: left panel : ×100; right panel : ×400. ( C – H ) MMP7 concentration: 80 ng/mL; ICG-001 concentration: 25 μM. Data are presented as mean ± SD.

Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

Techniques: Expressing, Cell Culture, Western Blot, Immunofluorescence, Staining, Luciferase, Activity Assay, Enzyme-linked Immunosorbent Assay, Immunostaining, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

doi: 10.3390/ijms27052209

Figure Lengend Snippet: Fibrosis progression in chronic BA mice was accompanied by increased MMP7 expression and EMT. Liver and serum samples of the control group (i.p. saline) and chronic BA group (i.p. RRV+ anti-Ly6G) were collected on days 14, 21, and 42 postnatally ( n = 5~7 mice in each group). ( A ) Representative micrograph of Masson trichrome staining of the liver from control and chronic BA mice. Quantification of collagen staining in the liver of control and chronic BA mice. Magnification: ×40. ( B , C ) Serum level and hepatic mRNA expression of MMP7 in control and chronic BA mice. ( D ) Representative images of immunostaining for MMP7 expression in liver (Days 42). ( E ) Representative images of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in liver (Days 42). ( F ) Serum level of sE-cadherin in mice ( n = 5 mice in each group). ( G ) Representative images of immunostaining β-catenin in cholangiocytes of mice. Magnification: left panel : ×100; right panel : ×400. Data are presented as mean ± SD.

Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

Techniques: Expressing, Control, Saline, Staining, Immunostaining

Journal: International Journal of Molecular Sciences

Article Title: Matrix Metalloproteinase 7 Mediates Epithelial–Mesenchymal Transition to Promote Liver Fibrosis Through E-cadherin/β-catenin Pathway in Biliary Atresia

doi: 10.3390/ijms27052209

Figure Lengend Snippet: MMP7 blockade attenuated liver fibrosis and EMT procession in chronic BA mice. ( A ) Schematic representation of chronic BA mice injected with anti-MMP7 antibody or IgG on days 21–25 and liver specimens collected on day 42 ( n = 5 mice in each group). ( B ) Representative micrographs of Masson trichrome stained liver tissue sections. Quantification of collagen staining in the liver. Magnification: ×40. ( C ) Representative images of immunostaining for EMT-related protein expression (including E-cadherin, Vimentin, S100A4) in the liver (Arrows indicate biliary epithelial cells). ( D ) Serum level of sE-cadherin in mice ( n = 5 mice in each group). ( E ) Representative images of immunostaining β-catenin in cholangiocytes of mice. ( F ) Serum level of MMP7 in mice ( n = 5 mice in each group). ( G ) Hepatic mRNA expression of MMP7 in mice ( n = 5 mice in each group). Magnification: left panel : ×100; right panel : ×400. Data are presented as mean ± SD.

Article Snippet: The activation of recombinant human MMP7 protein (R&D Systems, Minneapolis, MN, USA) was achieved through incubation with 4-aminophenylmercuric acetate (APMA; Sigma, St. Louis, MO, USA).

Techniques: Injection, Staining, Immunostaining, Expressing

Journal: Antibodies (Basel, Switzerland)

Article Title: Characterization of Active MMP9 in Chronic Inflammatory Diseases Using a Novel Anti-MMP9 Antibody.

doi: 10.3390/antib12010009

Figure Lengend Snippet: Figure 1. Activation of MMP9 by distinct proteases in vitro. Western blots for pro-MMP9 (92 kDa, red) and active MMP9 (84 kDa, green) after incubation with active (A) MMP7, (B) MMP12, (C) pepsin A, and (D) plasmin. Below each lane is the MMP9 activity towards gelatin expressed as gelatin fluorescence (arbitrary units) per minute. DQ, dye-quenched; MMP, matrix metalloproteinase.

Article Snippet: Recombinant active MMP7, MMP12, pepsin A, or plasmin (R&D Systems) was titrated against recombinant pro-MMP9 in MMP9 reaction buffer (150 mM NaCl, 10 mM Hepes pH 7.5, 0.05% brij-035, and 5 mM CaCl2).

Techniques: Activation Assay, In Vitro, Western Blot, Incubation, Activity Assay

Journal: ERJ Open Research

Article Title: Matrix metalloproteinase-7 is increased in lung bases but not apices in idiopathic pulmonary fibrosis

doi: 10.1183/23120541.00191-2022

Figure Lengend Snippet: Matrix metalloproteinase-7 (MMP7) expression is increased in IPF compared to control tissue and greatest in areas of end-stage disease. a) Representative brightfield staining of MMP7 in formalin-fixed paraffin-embedded tissue from the apex and base of 12 patients with idiopathic pulmonary fibrosis (IPF) and 10 non-diseased control (NDC) donors whose lungs were unsuitable for transplantation. A total of 44 sections were analysed ( supplementary table S1 ). Staining specificity was assessed for every sample by omitting the MMP7 antibody on sequential sections. Inserts show regions in IPF tissue at higher resolution. Open arrows show MMP7 positivity in elastin fibres. Filled arrows indicate cytoplasmic and apical staining on respiratory epithelial cells. Scale bars: solid line 500 μm; double line 100 μm. b) From the IPF patients, sequential tissue sections were stained with haemotoxylin and eosin and assessed for extent of fibrosis by a qualified pathologist (G.A. Fishbein) and a Fibrosis Score (1=mild fibrosis, 2=moderate fibrosis/possible usual interstitial pneumonia (UIP), 3=favour UIP or 4=end-stage fibrosis) was applied. Summary of the analysis is found in supplementary table S2 . *p=0.0234, Wilcoxon paired t-test. c) Overall MMP7 positivity in each tissue section was graded on the following scale: 1=No or rare staining; 2=subset of positive cells observed/moderate staining; or 3=Yes staining. *p=0.0115 and ****p<0.0001, Mann–Whitney t-test, **p=0.0078 Wilcoxon paired t-test.

Article Snippet: To validate MMP7 antibody specificity and act as positive controls, epithelial A549 cell culture supernatant (ATCC, Manassas, VA, USA) and recombinant MMP7 protein (R&D Systems, Minneapolis, MN, USA) were used.

Techniques: Expressing, Staining, Formalin-fixed Paraffin-Embedded, Transplantation Assay, MANN-WHITNEY

Journal: ERJ Open Research

Article Title: Matrix metalloproteinase-7 is increased in lung bases but not apices in idiopathic pulmonary fibrosis

doi: 10.1183/23120541.00191-2022

Figure Lengend Snippet: Matrix metalloproteinase-7 (MMP7) protein levels are increased in IPF whole lung lysates from bases but not apices compared to control tissue. MMP7 levels in whole lung lysates of 12 idiopathic pulmonary fibrosis (IPF) patients and 11 non-diseased control (NDC) donors were quantified by immunoblot. a) A representative immunoblot showing matched (apex and base) samples from N=3 IPF and N=3 NDC donors is shown. MMP7 zymogen (inactive) is detected at 28 kDa (green bands) and GAPDH (red bands, 36 kDa). Culture supernatant from A549 epithelial cells and activated recombinant MMP7 (18 kDa) are shown as positive controls. b) The level of MMP7 zymogen relative to GAPDH was significantly higher in IPF apex tissue compared to IPF base tissue (n=11 pairs). The level of MMP7/GAPDH was elevated in IPF base (n=12 samples) compared to NDC base (n=11 samples). Between-group comparisons were performed with one-way ANOVA with Holm–Sidak's test for multiple comparisons. Within group comparison was performed with paired-t-test. One IPF base sample without an apex match is shown as a half-filled in square.

Article Snippet: To validate MMP7 antibody specificity and act as positive controls, epithelial A549 cell culture supernatant (ATCC, Manassas, VA, USA) and recombinant MMP7 protein (R&D Systems, Minneapolis, MN, USA) were used.

Techniques: Western Blot, Recombinant, Comparison

Journal: ERJ Open Research

Article Title: Matrix metalloproteinase-7 is increased in lung bases but not apices in idiopathic pulmonary fibrosis

doi: 10.1183/23120541.00191-2022

Figure Lengend Snippet: Matrix metalloproteinase-7 (MMP7) gene expression correlated with MMP7 protein levels and was highest in end-stage tissue. a) MMP7 gene levels and MMP7 protein levels correlated in 45 samples of lung tissue from the lung apex (open symbols) and base (closed symbols) of 12 patients with idiopathic pulmonary fibrosis (IPF, square symbols) and 11 non-diseased control (NDC, circle symbols) donors (Pearson R=0.521, 95% confidence interval 0.27–0.71). b) Matched lung tissue was assessed for both extent of fibrosis via a trained pathologist (G.A. Fishbein) and MMP7 gene levels using RNA seq in 12 IPF patients. Values for extent of fibrosis scoring are found in supplementary table S2 . *p=0.272, Kruskal–Wallis one-way ANOVA with Dunn's multiple comparisons test. c) MMP7 gene expression levels in bulk lung tissue from the apex and base of 20 IPF patients and 14 NDC donors was assessed by RNA seq. *: FDR <0.05; ****: FDR ≤0.0001. UIP: interstitial pneumonia; FC: fold change to first group mentioned; FDR: false discovery rate; NS: nonsignificant; RPKM: reads per kilobase million.

Article Snippet: To validate MMP7 antibody specificity and act as positive controls, epithelial A549 cell culture supernatant (ATCC, Manassas, VA, USA) and recombinant MMP7 protein (R&D Systems, Minneapolis, MN, USA) were used.

Techniques: Expressing, RNA Sequencing Assay

Journal: ERJ Open Research

Article Title: Matrix metalloproteinase-7 is increased in lung bases but not apices in idiopathic pulmonary fibrosis

doi: 10.1183/23120541.00191-2022

Figure Lengend Snippet: Multiplex immunohistochemistry of matrix metalloproteinase-7 (MMP7). Representative images of idiopathic pulmonary fibrosis (IPF) and non-diseased control (NDC) tissue stained simultaneously for MMP7 (pseudocoloured yellow), E-cadherin (orange), CD45 (red), CD31 (cyan) and α-smooth muscle actin (SMA, green). Cell nuclei are counterstained with Hoechst (blue). Left panels show tissue imaged at 20× magnification captured with the Vectra 3 Quantitative Pathology Imaging System. Triangles indicate blood vessels, diamonds mark fibroblastic foci and arrows point to MMP7 + cells. The diamond shows a non-cellular form (no nucleus) that was not considered an MMP7 + cell.

Article Snippet: To validate MMP7 antibody specificity and act as positive controls, epithelial A549 cell culture supernatant (ATCC, Manassas, VA, USA) and recombinant MMP7 protein (R&D Systems, Minneapolis, MN, USA) were used.

Techniques: Multiplex Assay, Immunohistochemistry, Staining, Imaging

Journal: ERJ Open Research

Article Title: Matrix metalloproteinase-7 is increased in lung bases but not apices in idiopathic pulmonary fibrosis

doi: 10.1183/23120541.00191-2022

Figure Lengend Snippet: Matrix metalloproteinase-7 (MMP7 + ) epithelial cells are increased and correlated with MMP7 gene expression in IPF lung tissue. Formalin-fixed paraffin-embedded (FFPE) lung tissue from the apex and base of three patients with idiopathic pulmonary fibrosis (IPF) and from the apex of three non-diseased controls were stained sequentially with antibodies specific for MMP7, E-cadherin, CD45, CD31 and α-smooth muscle actin. Nuclei were stained with Hoescht dye. Positive staining was visualised with OPAL dyes (Perkin Elmer) and images were captured at 20× magnification using the Vectra 3 Quantitative Pathology Imaging System. a) The number of MMP7 + cells, b) MMP7 + /E-cadherin + and c) MMP7 + /E-cadherin − /CD45 − /CD31 − /α-smooth muscle actin − (elseNeg) cells are presented as a percentage of the total number of cells analysed. There was no difference between the groups. Absolute numbers of phenotyped cells can be found in supplementary figure S4D . The MMP7 gene expression level in matched samples correlates with d) the percentage of MMP7 + cells (Spearman R=0.74, p=0.0268), e) the percentage of MMP7 + /E-cadherin cells + (Spearman's R=0.73, p=0.0311) and f) percentage of MMP7 + /elseNeg cells (Spearman's R=0.83, p=0.0093). There was no difference in g) total number of cells quantified, h) mean area analysed or i) the percentage of E-cadherin + cells between the groups.

Article Snippet: To validate MMP7 antibody specificity and act as positive controls, epithelial A549 cell culture supernatant (ATCC, Manassas, VA, USA) and recombinant MMP7 protein (R&D Systems, Minneapolis, MN, USA) were used.

Techniques: Expressing, Formalin-fixed Paraffin-Embedded, Staining, Imaging

Journal: ERJ Open Research

Article Title: Matrix metalloproteinase-7 is increased in lung bases but not apices in idiopathic pulmonary fibrosis

doi: 10.1183/23120541.00191-2022

Figure Lengend Snippet: The epithelial transcriptomic profile of IPF lung bases is significantly different to apices. Matrix metalloproteinase-7 (MMP7) and 19 other epithelial genes were pre-selected for comparison. Heatmaps showing the a) averaged and b) individual expression levels of the 20 epithelial-related genes. Black arrows denote MMP7 bands. The log fold changes of the 20 genes in NDC base, IPF apex and IPF base tissue compared to NDC apex can be found in supplementary table S3 . IPF: idiopathic pulmonary fibrosis; NDC: non-diseased control; RPKM: reads per kilobase million.

Article Snippet: To validate MMP7 antibody specificity and act as positive controls, epithelial A549 cell culture supernatant (ATCC, Manassas, VA, USA) and recombinant MMP7 protein (R&D Systems, Minneapolis, MN, USA) were used.

Techniques: Comparison, Expressing

Journal: Frontiers in Oncology

Article Title: Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer

doi: 10.3389/fonc.2021.591893

Figure Lengend Snippet: Primers and their sequences for PCR analysis.

Article Snippet: For the MMP7 protein, the anti-MMP7 polyclonal antibody (1: 500 dilution, Proteintech, USA) was used.

Techniques: Sequencing

Journal: Frontiers in Oncology

Article Title: Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer

doi: 10.3389/fonc.2021.591893

Figure Lengend Snippet: Hub gene validation. (A) Western blotting expression of CDH3, LEF1, and MMP7 in the normal control (NC) and gastric cancer (GC) groups. (B) Relative expression of MMP7 by RT-qPCR analysis. P<0.05, compared with control. (C) Relative expression of CDH3 by RT-qPCR analysis. P<0.05, compared with control. (D) Relative expression of LEF1 by RT-qPCR analysis. P<0.05, compared with control.

Article Snippet: For the MMP7 protein, the anti-MMP7 polyclonal antibody (1: 500 dilution, Proteintech, USA) was used.

Techniques: Biomarker Discovery, Western Blot, Expressing, Control, Quantitative RT-PCR

Journal: Frontiers in Oncology

Article Title: Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer

doi: 10.3389/fonc.2021.591893

Figure Lengend Snippet: (A) The ROC of three genes for gastric cancer. (B) The detection of MMP7, CDH3, and LEF1 in the gastric tissues by immunohistochemical staining with their own antibodies. (200X, 400X).

Article Snippet: For the MMP7 protein, the anti-MMP7 polyclonal antibody (1: 500 dilution, Proteintech, USA) was used.

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Oncology

Article Title: Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer

doi: 10.3389/fonc.2021.591893

Figure Lengend Snippet: (A) Quantitative comparison of MMP7 expression between the two groups. (B) Quantitative comparison of CDH3 expression between the two groups. (C) Quantitative comparison of LEF1 expression between the two groups. (D) The linear correlation between CEA and the relative expression of MMP7. (E) The linear correlation between CEA and the relative expression of CDH3. (F) The linear correlation between CEA and the relative expression of LEF1.

Article Snippet: For the MMP7 protein, the anti-MMP7 polyclonal antibody (1: 500 dilution, Proteintech, USA) was used.

Techniques: Comparison, Expressing

Journal: Frontiers in Oncology

Article Title: Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer

doi: 10.3389/fonc.2021.591893

Figure Lengend Snippet: The correlation and linear regression analysis between GC and relevant gene expression.

Article Snippet: For the MMP7 protein, the anti-MMP7 polyclonal antibody (1: 500 dilution, Proteintech, USA) was used.

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer

doi: 10.3389/fonc.2021.591893

Figure Lengend Snippet: (A) The neural network prediction model of gastric cancer. The best training performance was 0.033031 at epoch 2678. (B) The predicted value of the data was verified against the actual value. (C) Residual plot of difference between actual and predicted values. (D) The final training model of neural network prediction model, and the relativity was 0.9624. (E) The high-risk warning range of gastric cancer at the level of the planform. (F) The high-risk warning range of gastric cancer at the level of the three-dimensional stereogram. The color represents MMP7 expression: “yellow” represents “high” and “blue” represents “low”.

Article Snippet: For the MMP7 protein, the anti-MMP7 polyclonal antibody (1: 500 dilution, Proteintech, USA) was used.

Techniques: Expressing